Research

My research focuses on understanding the mechanisms of herpes simplex virus (HSV) latent infection in neurons, with a particular emphasis on how neuronal function influences viral gene expression, replication, and reactivation. HSV establishes lifelong latency in sensory neurons, periodically reactivating to cause recurrent disease. While viral factors controlling latency and reactivation have been extensively studied, the role of host neuronal physiology in modulating these processes remains less understood.

I hypothesize that intrinsic neuronal properties—such as electrical activity, metabolic state, and signaling pathways—directly impact HSV gene expression, latency maintenance, and reactivation. By employing advanced neuronal culture models, live-cell imaging, animal models, and a variety of molecular virology techniques, my work aims to elucidate how neuronal function regulates viral persistence. A deeper understanding of these interactions may reveal novel therapeutic strategies to suppress reactivation and reduce HSV-associated morbidity. This research is supported by funding from the NINDS/NIH (R01NS138288). 

In a complementary line of research, I investigate the application of optogenetics combined with adeno-associated virus (AAV) vectors for precise gene delivery and manipulation in the brain. AAVs are a powerful tool for neuroscience due to their high transduction efficiency and cell-type specificity. My work explores strategies to achieve layer-specific gene expression in the cortex, which is critical for dissecting neural circuits with high precision.

By engineering AAV serotypes and promoters, as well as optimizing delivery methods, I aim to enhance the spatial and temporal control of transgene expression in defined cortical layers. This research has broad implications for both basic neuroscience—enabling precise interrogation of neural circuits—and therapeutic development, such as targeted gene therapy for neurological disorders. This research is supported by funding from the NIMH/NIH (U24MH137478).